|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5522981||1546065||2017||5 صفحه PDF||سفارش دهید||دانلود کنید|
- The main objective of the current study was to investigate the effect of L-carnitine supplementation on the developmental potential of camel oocytes.
- The recovered oocytes were inÂ vitro matured in l-carnitine treated media and the rates of maturation, fertilization and cleavage were recorded.
- For assaying the effect of l-carnitine on IVC, the recovered oocytes matured in l-carnitine free medium, fertilized and cultured in l-carnitine treated media.
- The present study concluded that, L-carnitine supplementation at the level of 0.5Â mg/ml concentration in either maturation or culture media tends to enhance the nuclear maturation and the developmental potential of camel oocytes.
The aim of the present study was to investigate the effect of L-carnitine (LC) addition during either IVM or IVC on the developmental potential of camel oocytes. In Experiment 1; camel oocytes were matured in the absence (control) or presence of different concentrations of LC (0.25Â mg, 0.5Â mg, 0.75Â mg and 1Â mg/ml) for 30Â h followed by inÂ vitro fertilization and culture up to blastocyst stage. Our results demonstrated that oocytes treated with 0.5Â mg/ml LC showed higher (PÂ <Â 0.05) rates of maturation (74.7%) and fertilization (62.2%) compared with control group, 0.25 and 1Â mg/ml of LC (60.2, 63.9, 59.7; 46.2, 48.7, 47.6%, respectively). Addition of 0.5Â mg/ml of LC to IVM medium improved the rates of cleavage and embryo development (morula and blastocyst) than those obtained in the control group, 0.25 and 1Â mg/ml of LC. No significant differences were noticed between 0.5 and 0.75Â mg/ml of LC supplemented groups in term of maturation, fertilization and culture. In Experiment 2; zygotes resulting from inÂ vitro matured (without LC) and fertilized were cultured in embryo culture medium supplemented with different concentrations of LC (0.25Â mg, 0.5Â mg, 0.75Â mg and 1Â mg/ml) or without LC (control). Also, the results showed a higher developmental rates to morula and blastocyst stages while adding L-carnitine at a level of 0.5 or 0.75Â mg/ml concentration in the culture medium during IVC when compared with other groups. In conclusion, the results demonstrated the usefulness of L-carnitine supplementation at the level of 0.5Â mg/ml during IVM or IVC after on the developmental potential of camel oocytes.
Journal: Theriogenology - Volume 104, December 2017, Pages 18-22