|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523018||1546064||2018||9 صفحه PDF||سفارش دهید||دانلود کنید|
- We optimized the canine oviduct epithelia cell collection method.
- We cultured canine oviduct epithelial cell culture with M171 medium.
- Coculture canine immature oocytes with oviduct epithelia cell increased oocyte maturation and development after activation.
Canine-assisted reproductive techniques have been successful for several years; however, the lack of an oocyte inÂ vitro maturation system has limited their application. The aim of this study was to evaluate the effect of canine oviduct epithelial cells (cOECs) on canine oocyte maturation inÂ vitro. Specifically, the method used for isolation of cOECs did not affect the expression of epithelial markers, E-cadherin and cytokeratin, on fresh, cultured and cryopreserved cells. Moreover, BrdU analysis showed that cOECs cultured in Medium 171 supplemented with mammary epithelial growth supplement were more proliferative than counterparts in advanced Dulbecco's modified Eagle medium or Medium 199. Maturation rate of canine oocytes collected from bitches at diestrus was significantly increased when oocytes were co-cultured with either fresh, cultured or frozen/thawed cOECs (13.23Â Â±Â 1.15%, 10.38Â Â±Â 4.89%, or 10.54Â Â±Â 2.96%, respectively) than that of control oocytes cultured without cOECs (2.48Â Â±Â 2.16%, pÂ <Â 0.05). Additionally, the number of oocytes collected from bitches at estrus the reached metaphase II was increased â¼4 fold in co-culture with fresh, cultured, or frozen/thawed cOECs (47.2Â Â±Â 3.82%, 45.4Â Â±Â 7.34%, and 46.9Â Â±Â 1.51%, respectively) as compared with oocytes cultured without cOECs (11.9Â Â±Â 3.18%, pÂ <Â 0.05). Nuclear maturation was further confirmed by assessing the formation of normal metaphase-II spindles, whereas cytoplasmic maturation was confirmed by inducing parthenogenetic oocyte activation. Embryonic development to the 8-cell stage was similar between inÂ vivo and inÂ vitro matured oocytes. These results suggested that co-culturing immature canine oocytes with cOECs facilitated canine oocyte maturation and early stages of embryonic development.
Journal: Theriogenology - Volume 105, 1 January 2018, Pages 66-74