|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523028||1546064||2018||6 صفحه PDF||سفارش دهید||دانلود کنید|
- Microarrays analysis showed that in placentomes of cows with retained fetal membranes mRNA of 'modern' PAGs is higher expressed than in controls.
- Immunohistochemistry showed that in retained fetal membranes the number of PAG-positive binucleate trophoblast giant cells is significantly higher than in controls.
- PAG concentrations in maternal serum at parturition did not differ significantly between cows with RFM and controls.
In cows, retained fetal membranes (RFM) are a major problem in reproduction. The timely detachment of fetal membranes after parturition requires well coordinated maturation processes in the placenta. One feature of placental maturation in cows is a prepartal decline in the number of binucleate trophoblast giant cells (BNC) in the fetal chorion. Pregnancy associated glycoproteins (PAGs) are a group of proteins, produced by trophoblast cells in artiodactyls. We studied aspects of PAG expression in cows with and without RFM. The numerical density of PAG-positive immunostained BNC in placentomal samples, collected from cows with normal expulsion of fetal membranes (nÂ =Â 20) and cows with RFM (nÂ =Â 20) was determined. The number of PAG-positive BNCs was significantly higher in cows with RFM, compared to controls. The concentration of PAGs in maternal serum in prepartum, intrapartum, and postpartum cows was measured (RFM nÂ =Â 20; controls nÂ =Â 68). No significant differences between RFM and controls were detected. Microarray analysis of placental PAG mRNA expression was done with two types of microarrays: Affymetrix (RFM nÂ =Â 20; controls nÂ =Â 20) and Agilent (RFM nÂ =Â 8; controls nÂ =Â 8). Both microarrays showed a significantly higher expression of modern PAGs in RFM cases.Our results show that the expression of modern PAGs, which are produced by BNCs and are secreted into the maternal organism, are differentially expressed in RFM. Although the concentration in peripheral maternal blood did not differ between RFM and controls, the local concentration in the placenta is likely to be higher in RFM cases. This suggests the possibility of local regulatory roles of PAG in the release of bovine fetal membranes.
Journal: Theriogenology - Volume 105, 1 January 2018, Pages 158-163