|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5523031||1546064||2018||4 صفحه PDF||سفارش دهید||دانلود کنید|
- SpayCheck® is a reliable test to determine AMH concentrations in alpacas.
- There is no difference in AMH concentrations between pre-pubertal and adult males. This warrants further investigation.
- In adult males, AMH concentrations decrease significantly following castration.
- The measurement of AMH can be used to differentiate geldings displaying male behavior from cryptorchids alpacas.
The synthesis of anti-Müllerian Hormone (AMH) by the Sertoli cells in males is crucial for sexual differentiation. There are no studies on AMH in Camelids. The objectives of this research were to 1) compare AMH serum concentrations in prepubertal and adult male alpacas and 2) determine the effect of castration on these concentrations in adult males to provide a validation of a commercial AMH test in alpacas. Serum samples were obtained from 15 prepubertal animals (5 for each age groups of 6, 7 and 8 months) and from 5 adult males (age 5-9 years), pre- and 24Â h post-castration. AMH was determined with a quantitative ELISA according to the manufacture's instructions. There was not significant difference (PÂ <Â 0.05) in AMH level (pg/ml) between pre-pubertal (549.9Â Â±Â 120.8, 789.4Â Â±Â 172.3, 597.5Â Â±Â 177.3 for ages 4, 7, and 8 months, respectively) and adult alpacas (938.7Â Â±Â 175.9). In adult males, AMH concentration decreased significantly following castration (PÂ <Â 0.05) (938.7Â Â±Â 383.5Â pg/ml) pre-castration, and 222.1Â Â±Â 116.5Â pg/ml) after castration). There was a positive correlation between testosterone levels and AMH. In conclusion, the quantitative assay used is a reliable test to determine AMH in alpacas. The AMH level in prepubertal and adult alpacas appear to not differ, contrarily from other mammals, this requires further investigation. The decrease in serum AMH concentrations after castration suggests that measurement of this hormone can be used to diagnose bilateral cryptorchid or hemicastrated unilateral cryptorchid animals in this species.
Journal: Theriogenology - Volume 105, 1 January 2018, Pages 174-177