کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5527250 | 1401573 | 2017 | 8 صفحه PDF | دانلود رایگان |
- miR-486 is a rapid response miRNA to hypoxia in erythroleukemia cells.
- Exosomal miR-486 regulates the erythroid differentiation of TF-1 and cord blood CD34+ cells.
- Sirt1 inhibition contributes to miR-486-induced erythroid differentiation.
MicroRNAs (miRNAs) regulate the hypoxia-induced erythroid differentiation of hematopoietic cells. In this study, we identified that miR-486 was a rapid response miRNA to hypoxia in erythroleukemia TF-1 cells. Hypoxia exposure increased both intracellular and miR-486 levels of TF-1 cells. Ectopic miR-486 expression enhanced the growth and erythroid differentiation of TF-1 cells, whereas miR-486 inhibition suppressed their growth and erythroid differentiation. Treatment of TF-1 and cord blood CD34+ cells with exogenous containing miR-486 resulted in an increase of intracellular miR-486 level and enhanced erythroid differentiation. Furthermore, we identified that Sirt1 is a miR-486 target gene which modulates hypoxia-induced erythroid differentiation of TF-1 cells. Thus we identified a novel miRNA regulatory network that contributes to hypoxia-induced erythroid differentiation of hematopoietic cells.
Journal: Experimental Cell Research - Volume 351, Issue 1, 1 February 2017, Pages 74-81