کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5528752 | 1548555 | 2017 | 5 صفحه PDF | دانلود رایگان |
- Bisdemethoxycurcumin (BDMC) enhances X-ray-induced apoptosis.
- BDMC enhances X-ray-induced dephosphorylation of Bcl-2's FLD region.
- BDMC facilitates X-ray-induced p53/Bcl-2 interaction.
- BDMC potentially induces a neutralization of Bcl-2's anti-apoptotic function.
Bisdemethoxycurcumin (BDMC), which is isolated from the rhizomes of Curcuma longa, has anti-inflammatory and anti-carcinogenic activities. Here we found that BDMC enhanced X-ray-induced apoptosis in human T-cell leukemia MOLT-4 cells. Knockdown of p53 significantly attenuated the radiosensitizing effect of BDMC. However, BDMC did not enhance X-ray-mediated activation of the p53 signaling pathway via p53's transactivation or mitochondrial translocation. On the other hand, BDMC promoted the X-ray-induced dephosphorylation at Ser 70 in Bcl-2's flexible loop regulatory domain and Bcl-2 binding to p53. Overexpressing Bcl-2 completely blocked the BDMC's radiosensitization effect. Our results indicate that BDMC stimulates the dephosphorylation and p53-binding activity of Bcl-2 and suggest that BDMC may induce a neutralization of Bcl-2's anti-apoptotic function, thereby enhancing X-ray-induced apoptosis.
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 815, March 2017, Pages 1-5