No evidence of DNA damage by co-exposure to extremely low frequency magnetic fields and aluminum on neuroblastoma cell lines

- Both ELF-MF and aluminum are widespread environmental pollutants.
- Whether co-exposure to ELF-MF and Al is harmful to cells is unknown.
- Testing early effects of ELF-MF and Al co-exposure in SH-SY5Y and SK-N-BE-2 cells.
- Short-term ELF-MF and Al co-exposure does not have harmful effects on human neuroblastoma cells.

Whether exposure to 50-60 Hz extremely low frequency magnetic fields (ELF-MF) exerts neurotoxic effects is a debated issue. Analogously, the potential role of Aluminum (Al) in neurodegeneration is a matter of controversial debate. As all living organisms are exposed to ELF-MF and/or Al daily, we found investigating the early effects of co-exposure to ELF-MF and Al in SH-SY5Y and SK-N-BE-2 human neuroblastoma (NB) cells intriguing.SH-SY5Y5 and SK-N-BE-2 cells underwent exposure to 50 Hz ELF-MF (0.01, 0.1 or 1 mT) or AlCl3 (4 or 40 μM) or co-exposure to 50 Hz ELF-MF and AlCl3 for 1 h continuously or 5 h intermittently. The effects of the treatment were evaluated in terms of DNA damage, redox status changes and Hsp70 expression. The DNA damage was assessed by Comet assay; the cellular redox status was investigated by measuring the amount of reduced glutathione (GSH) and glutathione disulfide (GSSG) while the inducible Hsp70 expression was evaluated by western blot analysis and real-time RT-PCR.Neither exposure to ELF-MF or AlCl3 alone induced DNA damage, changes in GSH/GSSG ratio or variations in Hsp70 expression with respect to the controls in both NB cell lines. Similarly, co-exposure to ELF-MF and AlCl3 did not have any synergic toxic effects.The results of this in vitro study, which deals with the effects of co-exposure to 50 Hz MF and Aluminum, seem to exclude that short-term exposure to ELF-MF in combination with Al can have harmful effects on human SH-SY5Y and SK-N-BE-2 cells.