کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530678 1549381 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperOsteopontin exacerbates Pseudomonas aeruginosa-induced bacteremia in mice
ترجمه فارسی عنوان
استئوپونتین باکتریایی ناشی از سودوموناس آئروژینوزا را در موش ها افزایش می دهد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


- OPN-KO mice exhibited reduced mortality with P. aeruginosa-induced bacteremia compared with WT mice.
- OPN exacerbated spleen bleeding and functional impairment.
- OPN promotes secretion of pro-inflammatory cytokines.
- Percentages and absolute numbers of B cells were increased in spleens of OPN-KO mice.

Osteopontin (OPN) is a multifunctional protein involved in various pathophysiological processes. However, the role of OPN in Pseudomonas aeruginosa-related sepsis is not yet clear. Here, we found that OPN expression was elevated in plasma and spleen samples from P. aeruginosa-infected mice. To determine the function of OPN in sepsis, we used wild-type (WT) and OPN-knockout (KO) mice with P. aeruginosa-induced bacteremia. We found that OPN-KO mice exhibited reduced mortality compared with WT mice and that OPN exacerbated spleen bleeding and functional impairment. OPN-KO mice exhibited reduced secretion of pro-inflammatory cytokines, such as interferon-γ, interleukin (IL)-1β, IL-12, and tumor necrosis factor-α, whereas levels of anti-inflammatory cytokine IL-10 and the leukocyte trafficking mediator macrophage inflammatory protein (MIP)-2 were not altered. Additionally, the percentages and absolute numbers of B cells were elevated in the spleens of OPN-KO mice. Thus, OPN promoted sepsis in P. aeruginosa-infected mice and potentially blocked B cell-dependent immunity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 318, August 2017, Pages 23-28
نویسندگان
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