کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5530705 1549384 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research paperBovine peripheral blood WC1+ and WC1neg γδ T lymphocytes modulate monocyte-derived macrophage effector functions during in vitro Mycobacterium avium subspecies paratuberculosis infection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Research paperBovine peripheral blood WC1+ and WC1neg γδ T lymphocytes modulate monocyte-derived macrophage effector functions during in vitro Mycobacterium avium subspecies paratuberculosis infection
چکیده انگلیسی


- The percentage of circulating γδ T cells is inversely proportional to cattle age.
- Map viability is reduced when Map-infected MDMs are co-cultured with autologous peripheral blood WC1− or WC1+ γδ T cells of calves.
- WC1− γδ T cells produce more IFN-γ than WC1+ γδ T cells when co-cultured with Map-infected-MDMs.

The importance of bovine γδ T lymphocytes during anti-mycobacterial immunity is recognized; however, the role of major subsets of γδ T lymphocytes (WC1+ and WC1neg) in this process remains unclear. We investigated how WC1+ and WC1neg γδ T lymphocyte subsets of calves modulate monocyte-derived macrophage (MDM) functions during Map infection in vitro. To achieve this, Map-infected or uninfected MDMs from young calves were co-cultured with autologous WC1+ or WC1neg γδ T lymphocytes. Our data indicate that WC1+ and WC1neg γδ T lymphocytes of young calves modulate effector functions of MDMs with respect to Map killing, CD11b and MHC-II expression. We observed differences in IFN-γ production and CD25 expression on γδ T lymphocyte subsets, as well as MDM expression of CD1b when in contact with WC1neg γδ T lymphocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 315, May 2017, Pages 34-44
نویسندگان
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