کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5531695 | 1401808 | 2017 | 13 صفحه PDF | دانلود رایگان |
- A novel high-throughput method for spatial cis-regulatory analysis is developed.
- The method uses quantitative profiles of single-embryo resolution reporter assays.
- The method can rapidly predict germ layer-specific CRM activities.
- The method can detect spatial responses of CRMs to gene perturbations.
- The method can be scaled up to many hundreds of CRMs in a single experiment.
Cis-regulatory modules (CRMs) control spatiotemporal gene expression patterns in embryos. While measurement of quantitative CRM activities has become efficient, measurement of spatial CRM activities still relies on slow, one-by-one methods. To overcome this bottleneck, we have developed a high-throughput method that can simultaneously measure quantitative and spatial CRM activities. The new method builds profiles of quantitative CRM activities measured at single-embryo resolution in many mosaic embryos and uses these profiles as a 'fingerprint' of spatial patterns. We show in sea urchin embryos that the new method, Multiplex and Mosaic Observation of Spatial Information encoded in Cis-regulatory modules (MMOSAIC), can efficiently predict spatial activities of new CRMs and can detect spatial responses of CRMs to gene perturbations. We anticipate that MMOSAIC will facilitate systems-wide functional analyses of CRMs in embryos.
Journal: Developmental Biology - Volume 422, Issue 2, 15 February 2017, Pages 92-104