کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534199 1550836 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Androgens and androgen receptor signaling contribute to ovarian development in the chicken embryo
ترجمه فارسی عنوان
سیگنالینگ آندروژن ها و گیرنده آندروژن به رشد تخمدان در جنین مرغ کمک می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی


- The AR expression is higher in female than male in embryonic gonads of chicken.
- Nuclear localization is observed in cortical cords and lacunae in female.
- Embryonic male gonads exhibits cytoplasmic localization.
- Female ARKD embryos shows decrease of lacunae and disruption in the cortical cords.
- Androgen and AR signaling essential for development of embryonic ovary in chciken.

Androgens and androgen receptor (AR) signaling play important roles throughout development. In the chicken, AR signaling is involved in reproduction; however, its specific role is unclear. We show that AR signaling is involved in the normal development of the female embryonic gonads. The AR mRNA level was detected in male and female embryonic gonads by quantitative RT-PCR, and its expression was higher in females than in males at all developmental stages examined. In female embryos, the AR localized to nuclei of cells in the left gonad. Although AR expression was low in the majority of the medulla, high expression was detected in cells of lacunae within the medulla. In addition, AR expression increased in cells of cortical cords within the cortex with the progression of development. AR expression in the right gonad was lower than that in left gonad throughout development. In the male gonad, the AR localized to the cytoplasm of cells in seminiferous tubules at all stages. Female AR knockdown (ARKD) embryos infected with a retrovirus expressing micro RNAs targeting the AR showed normal asymmetric gonads (development of the left and depression of the right gonads), whereas the number of lacunae decreased. Furthermore, there was a disruption in the structure of cortical cords. By contrast, the gonads of ARKD males developed normally during embryogenesis. These results indicate that androgens and AR signaling are essential for the development of lacunae and cortical cords in gonads of female embryos.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 443, 5 March 2017, Pages 114-120
نویسندگان
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