In vitro characterization and endocrine regulation of cholesterol and phospholipid transport in the mammary gland

- MAC-T cells are a suitable model for lipid transport studies in the mammary gland.
- Cholesterol and phospholipid transport in MEC is mediated by apoA-1/ABCA1 and ABCG1/HDL dependent pathways.
- ApoA-1/ABCA1 activity in MEC causes activation of intracellular signaling cascades (JAK-2, MAPK).
- The transported cholesterol stems mainly from the plasma membrane.
- Insulin plays an important role in regulating lipid transport in MEC.

Cell-based studies previously showed that the ATP-binding cassette transporter A1 (ABCA1) transfers cholesterol across mammary epithelial cells (MEC). Data for phospholipid transport are lacking, and it is unclear from which cellular source the transported cholesterol stems, whether this transport activates signaling pathways, and how lactogenic hormones regulate it. To clarify these aspects, lipid transport and expressional analyses were performed in bovine primary (bMEC) and/or immortalized (MAC-T) MEC cultures. Lipid efflux and ABCA1, ABCG1 and liver X receptorα mRNA levels were higher in MAC-T than bMEC. In MAC-T, the transported cholesterol originated mainly from the plasma membrane. ABCA1 dependent cholesterol efflux was higher than phosphatidylcholine efflux, was suppressed by probucol (ABCA1 inhibitor), AG490 (janus kinase-2 inhibitor), PD98059 (mitogen activated protein kinase kinase inhibitor) and pretreatment with β-cyclodextrin (lowering membrane cholesterol). Insulin was the only hormone significantly increasing cholesterol efflux. In conclusion, this study gives novel mechanistic and regulatory insights into the transport of cholesterol and phospholipids in MEC.