|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5534501||1402182||2017||5 صفحه PDF||سفارش دهید||دانلود کنید|
- A real-time PCR assay to detect Bartonella spp. species has been developed.
- This assay has been tested with the most frequent species of Bartonella spp.
- Its flexibility may allow to detect more species, even those not described yet.
A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100Â ng-100Â fg and sensitivity was 50 copies/reaction.
Journal: Molecular and Cellular Probes - Volume 32, April 2017, Pages 55-59