کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5534501 1551171 2017 5 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Short communicationDevelopment of a real-time PCR for Bartonella spp. detection, a current emerging microorganism
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Short communicationDevelopment of a real-time PCR for Bartonella spp. detection, a current emerging microorganism
چکیده انگلیسی


- A real-time PCR assay to detect Bartonella spp. species has been developed.
- This assay has been tested with the most frequent species of Bartonella spp.
- Its flexibility may allow to detect more species, even those not described yet.

A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 32, April 2017, Pages 55-59
نویسندگان
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