کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5534623 1551273 2017 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microglia derived from the axotomized adult rat facial nucleus uptake glutamate and metabolize it to glutamine in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Microglia derived from the axotomized adult rat facial nucleus uptake glutamate and metabolize it to glutamine in vitro
چکیده انگلیسی


- Axotomized rat facial nucleus-derived microglia were isolated and cultured in vitro.
- The microglia were sub-cultured non-invasively by a specific polymer-grafted dish.
- The microglia showed a specific uptake of 14C-Glu by a glutamate transporter, GLT-1.
- The microglia expressed glutamine synthetase (GS) protein and showed GS activity.
- The microglia were suggested to change the uptaken 13C-Glu to 13C-Gln by their GS.

Microglia in the axotomized adult rat facial nucleus (axoFN) have been shown to highly express a glutamate transporter (GLT-1). The microglia appear to serve as glutamate (Glu) scavengers in the axoFN. However, there is no evidence that the microglia actually have the ability to uptake Glu and convert it to Gln. In this study, we investigated whether axoFN-derived microglia (axoFN-microglia) can uptake Glu and metabolize it to Gln. Microglia obtained by explant culture of axoFN on poly(N-isopropylacrylamide)-grafted dishes were non-invasively sub-cultured onto dishes or wells. Immunoblotting and Glu-uptake experiments revealed that the axoFN-microglia uptake 14C-Glu mainly by GLT-1 activity. Immunoblotting and immunocytochemical methods clarified that axoFN-microglia express the Gln synthetase (GS) protein in the same manner as newborn rat brain-derived primary microglia (NRB-microglia). Biochemical analysis demonstrated that the specific activity of GS of axoFN-microglia is similar to that of NRB-microglia, suggesting that these microglia play equivalent roles in the metabolic conversion of Glu to Gln. Nuclear magnetic resonance analysis clarified that NRB-microglia metabolize [13C]Glu to [13C]Gln depending on the incubation time, inferring the similar potential of axoFN-microglia. Taken together, these results demonstrate that axoFN-microglia express functional GLT-1 and GS proteins, and are strongly suggested to serve as Glu scavengers in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 102, January 2017, Pages 1-12
نویسندگان
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