کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5535552 1551550 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Original ResearchMEM α Promotes Cell Proliferation and Expression of Bone Marrow Derived Equine Mesenchymal Stem Cell Gene Markers but Depresses Differentiation Gene Markers
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Original ResearchMEM α Promotes Cell Proliferation and Expression of Bone Marrow Derived Equine Mesenchymal Stem Cell Gene Markers but Depresses Differentiation Gene Markers
چکیده انگلیسی

Highlight
- Many media have been used to propagate BM-eMSC but there is a lack of comparative reports between the commercial media for eMSCs. In this study, we aimed to compare five basic commercial culture media to expand BM-eMSCs by measuring proliferation rate and expression of MSC gene markers and differentiation markers.
- Our results were concluded that MEM α is the best culture media in which to expand BM-eMSCs up to 14 days, compared to DMEM-LG, DMEM-HG, RPMI-1640, and DMEM/F12. It promotes rapid cell proliferation, maintains high expression of BM-eMSC genes, but depresses differentiation gene markers. MEM α should be used as recommended medium for in vitro expansion of BM-eMSCs for cell transplantation studies.

Equine bone marrow-derived mesenchymal stem cells (BM-eMSCs) have been used in veterinary clinics and research worldwide. For clinical use, in vitro propagation of BM-eMSCs is required (at least 2 weeks) to yield sufficient cell number for transplantation. Many culture media have been used to propagate human and animal MSCs but there are very few comparative studies of equine mesenchymal stem cells (eMSCs). The best culture media must promote cell proliferation and maintain eMSC properties. The objective of this study was to compare five basic commercial culture media by examining the proliferation rate and a representative MSC gene expression profile. Five culture media Dulbecco's modified eagle medium (DMEM)-LG, DMEM-HG, minimum essential medium alpha (MEM α), RPMI-1640, and DMEM/F12 were compared. Cultured BM-eMSCs were collected at day 7 and day 14 to measure cell number and gene expression. Relative gene expression was performed using real-time RT-PCR. Our results showed that MEM α was significantly superior to other media (P < .05) in 14-day culture, enhancing the expression of eMSC genes (ITGB1, CD44 and POU5F1) but depressing differentiation genes (DCN, PPARG and ADIPOQ) in addition to promoting rapid cell proliferation when compared to the other media. We concluded that MEM α was the best media to propagate BM-eMSCs up to 14 days as it supported cell proliferation while maintaining eMSC gene expression. From this, we propose that MEM α may be the most suitable medium for short-term culture of BM-eMSCs for cell transplantation studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Equine Veterinary Science - Volume 50, March 2017, Pages 8-14
نویسندگان
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