کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5536246 1551700 2017 8 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Ionophore A23187 shows anti-tuberculosis activity and synergy with tebipenem
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI
فقط 3 هزار تومان
سفارش ترجمه تخصصی
با تضمین قیمت و کیفیت
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Ionophore A23187 shows anti-tuberculosis activity and synergy with tebipenem
چکیده انگلیسی

The objective of this study was to find molecules with anti-mycobacterial activity from a natural compounds library, investigate their mechanisms of resistance, and assess their synergy with antibiotics. We screened a library of 2582 natural compounds with Mycobacterium aurum with the aim of identifying molecules with anti-mycobacterial activity. The hits with the lowest MICs in M. aurum were also tested for their antimicrobial activity in other mycobacterial species including M. tuberculosis complex strains. The chequerboard titration assay was chosen for determining drug interactions in vitro. Spontaneous resistant mutants were isolated and their whole genome sequences compared to wild type and resistant mutants to identify resistance mechanisms. We found that ionophores show anti-mycobacterial activity in vitro. Resistance mechanism to ionophores is mediated by the MmpL5-MmpS5 transporter overexpression. Ionophore A23187 enhanced beta-lactam activity in M. tuberculosis infected macrophage. It will help in the investigation of new drug combinations against bacterial infections including tuberculosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tuberculosis - Volume 107, December 2017, Pages 111-118
نویسندگان
, , , , , , , , , ,
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI
فقط 3 هزار تومان
سفارش ترجمه تخصصی
با تضمین قیمت و کیفیت