کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5541925 | 1402512 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of the capillary electrophoresis method for measurement of immunoglobulin concentration in ewe colostrum
ترجمه فارسی عنوان
بررسی روش الکتروفورز مویرگی برای اندازه گیری غلظت ایمونوگلوبولین در شیر مادر
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کلمات کلیدی
چای سبز ایمونوگلوبولین، الکتروفورز مویرگی، ایمونوادفیوس رادیال،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Capillary electrophoresis (CE) is a technique routinely used in clinical laboratories that allows the separation and quantification of blood serum proteins in a rapid, precise, accurate, and inexpensive manner. Recently, CE has been proposed to separate and measure colostral proteins, but an evaluation of the agreement between CE and radial immunodiffusion (RID) method, currently used to quantify IgG in colostrum, is still lacking. The purpose of this study was to test the ability of a CE instrument, normally used in blood serum protein analysis, to realize the correct quantification of total Ig concentration in ewe colostrum, using RID assay as reference. Colostrum samples (n = 68) were collected from 35 multiparous Sarda ewes at first milking (n = 33) and at 24 h postpartum (n = 35). The mean ± standard deviation of IgG concentration measured by RID and whey colostrum total Ig concentration measured by CE were 54.76 ± 41.82 g/L and 54.70 ± 41.43 g/L, respectively. Lin's concordance correlation coefficient (r = 0.993; 95% confidence interval = 0.989 to 0.996) and linear regression analysis results (RID = 1.0022CE â 0.063; R2 = 0.986) showed an excellent agreement between these 2 methods. Bland-Altman analysis confirmed that CE method can be a suitable alternative to RID: the mean of the differences between CE and RID was â0.055 ± 4.95 g/L (95% confidence interval = â1.25 to 1.14 g/L) and the agreement limits were â9.75 to 9.60 g/L (low limit 95% confidence interval = â11.82 to â7.68 g/L; high limit 95% confidence interval = 7.57 to 11.72 g/L). In conclusion, the current study indicates that CE method may be a reliable tool for the quantification of the total Ig concentration in ewe colostrum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 100, Issue 8, August 2017, Pages 6465-6469
Journal: Journal of Dairy Science - Volume 100, Issue 8, August 2017, Pages 6465-6469
نویسندگان
V. Lopreiato, C. Ceniti, F. Trimboli, E. Fratto, M. Marotta, D. Britti, V.M. Morittu,