کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5546619 1402751 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii
چکیده انگلیسی

Mogrosides and steroid saponins are tetracyclic triterpenoids found in Siraitia grosvenorii. Squalene synthase (SQS) and cycloartenol synthase (CAS) are key enzymes in triterpenoid and steroid biosynthesis. In this study, full-length cDNAs of SgSQS and SgCAS were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction (RACE-PCR) approach. The SgSQS cDNA has a 1254 bp open reading frame (ORF) encoding 417 amino acids, and the SgCAS cDNA contains a 2298 bp ORF encoding 765 amino acids. Bioinformatic analysis showed that the deduced SgSQS protein has two transmembrane regions in the C-terminal. Both SgSQS and SgCAS have significantly higher levels in fruits than in other tissues, suggesting that steroids and mogrosides are competitors for the same precursors in fruits. Combined in silico prediction and subcellular localization, experiments in tobacco indicated that SgSQS was probably in the cytoplasm or on the cytoskeleton, and SgCAS was likely located in the nucleus or cytosol. These results will provide a foundation for further study of SgSQS and SgCAS gene functions in S. grosvenorii, and may facilitate improvements in mogroside content in fruit by regulating gene expression.

Mogrosides are the major components in fruits of Siraitia grosvenorii, which are a class of tetra-triterpenoids. In order to better illustrate the mogroside biosynthetic pathway in S. grosvenorii, two genes encoding SgSQS and SgCAS were selected for gene cloning, bioinformatic analysis and expression pattern analysis. All the results could be useful for engineering of triterpenoids and steroids or heterotrophic systems in the future.105

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Pharmaceutica Sinica B - Volume 7, Issue 2, March 2017, Pages 215-222
نویسندگان
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