کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5555380 1559747 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ponciretin attenuates ethanol-induced gastric damage in mice by inhibiting inflammatory responses
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Ponciretin attenuates ethanol-induced gastric damage in mice by inhibiting inflammatory responses
چکیده انگلیسی


- Ponciretin and poncirin inhibited IL-8 expression and NF-κB activation in ethanol- or LPS-stimulated KATO III cells
- Ponciretin and poncirin protected ethanol-induced gastric injury in mice
- Ponciretin and poncirin inhibited ethanol-induced secretion of CXCL-4 and IFN-γ in mice
- Ponciretin and poncirin inhibited ethanol-induced NF-κB activation in mice
- Anti-gastritis effect of ponciretin was greater than that of poncirin in vitro and in vivo.

BackgroundPoncirin (PO) and isosakuranetin (or ponciretin [PT]) are compounds found in fruits of the genus Citrus. They are frequently used in traditional Chinese medicine for the treatment of inflammation and asthma. Therefore, we examined their anti-gastritis effects in vitro and in vivo.MethodsThe anti-inflammatory effects of PO and PT were examined using ethanol- or LPS-stimulated KATO III cells. Gastritis was induced in ICR mice via intragastric injection of absolute ethanol. Levels of inflammatory markers were measured by enzyme-linked immunosorbent assay, immunoblotting, and quantitative polymerase chain reaction.ResultsTreatment with PT or PO inhibited the secretion of interleukin (IL)-8 and tumor necrosis factor (TNF) in ethanol- or LPS-stimulated KATO III cells. They also reduced the activation of nuclear factor kappa B (NF-κB). Pre-treatment with PT or PO significantly protected against ethanol-induced hemorrhagic gastritis, characterized by edema, tissue erosions, and mucosal friability in mice. Treatment with PT or PO suppressed ethanol-induced NF-κB activation and the release of TNF, IL-8, and IFN-γ. The protective effect of PT was greater than that of PO and comparable to ranitidine, a positive control.ConclusionPT may attenuate ethanol-induced gastritis by inhibiting the infiltration of immune cells, including neutrophils, via the regulation of CXCL4 (or IL-8) secretion and the activation NF-κB.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 43, February 2017, Pages 179-186
نویسندگان
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