کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5560971 | 1562072 | 2017 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Caffeine administration alters the behaviour and development of Galleria mellonella larvae
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کلمات کلیدی
PBSIAAFDRDTTTFARP-HPLCEC50LFQLabel free quantification - آزمایشی رایگانTrifluoroacetic acid - اسید Trifluoroaceticiodoacetic acid - اسید ید اسیدهای آلیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancestandard error - خطای استانداردdithiothreitol - دیتیوتریتولIn vivo screening - غربالگری در وینوPhosphate buffered saline - فسفات بافر شورMass Spec - مشخصات جرمImmunity - مصونیتfalse discovery rates - نرخ کشف کاذبhalf maximal effective concentration - نیمه حداکثر غلظت موثرCaffeine - کافئینGalleria - گالریا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effect of feeding caffeine on the behaviour and neural proteome of Galleria mellonella larvae was assessed. Caffeine was administered to larvae by force feeding and the metabolites theobromine and theophylline were subsequently detected by RP-HPLC analysis. Administration of caffeine to larvae resulted in reduced movement and a reduction in the formation of pupae. The production of the muscle relaxant theophylline may contribute to the reduction in larval movement. Analysis of the changes in proteome of the brain and surrounding tissues of caffeine fed larvae revealed an increase in the abundance of immune related proteins such as immune-related Hdd1 (6.28 fold increase) and hemolin (1.68 fold increase), ATPase associated proteins such as HÂ + transporting ATP synthase O subunit isoform 1 (1.87 fold increase) and HÂ + transporting ATP synthase delta subunit (1.53 fold increase) and proteins indicative of brain trauma such as troponin T transcript variant B, partial (1.55 fold increase). Proteins involved in development and protein degradation such as SUMO-activating enzyme subunit 1 (3.08 fold decrease) and chitin deacetylase, partial (3.67 fold decrease) were decreased in abundance. The results presented here indicate that caffeine is metabolised in a similar way in G. mellonella larvae to that in mammals and results in a variety of behavioural and developmental alterations. Utilisation of insects for studying the effects of caffeine and other neuroactive compounds may offer new insights into their mode of action and reduce the need to use mammals for this type of analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurotoxicology and Teratology - Volume 64, November 2017, Pages 37-44
Journal: Neurotoxicology and Teratology - Volume 64, November 2017, Pages 37-44
نویسندگان
Ronan Maguire, Martin Kunc, Pavel Hyrsl, Kevin Kavanagh,