کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5562486 | 1562699 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Manganese exposure: Linking down-regulation of miRNA-7 and miRNA-433 with α-synuclein overexpression and risk of idiopathic Parkinson's disease
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کلمات کلیدی
CCND1fibroblast growth factor 20NTRK3Fgf20Cyclin E1fibroblast growth factor 7CCNE1HMGA1GRB2Slc7a5Wnt3aCrkLFGF7Cyclin D1SH-SY5YPAK1Bcl2SNCAα-SynFOXO1B-cell CLL/lymphoma 2 - CLL / لنفوم B-cell 2α-synuclein - α-سینوکلینParkinson's disease - بیماری پارکینسونManganese - منگنز MiRNA - میکروRNA، ریزآرانای، miRNAGrowth factor receptor bound protein 2 - پروتئین متصل گیرنده عامل رشد 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Manganese is an essential trace element however elevated environmental and occupational exposure to this element has been correlated with neurotoxicity symptoms clinically identical to idiopathic Parkinson's disease. In the present study we chronically exposed human neuroblastoma SH-SY5Y cells to manganese (100 μM) and carried out expression profiling of miRNAs known to modulate neuronal differentiation and neurodegeneration. The miRNA PCR array results reveal alterations in expression levels of miRNAs, which have previously been associated with the regulation of synaptic transmission and apoptosis. The expressions of miR-7 and miR-433 significantly reduced upon manganese exposure. By in silico homology analysis we identified SNCA and FGF-20as targets of miR-7 and miR-433. We demonstrate an inverse correlation in expression levels where reduction in these two miRNAs causes increases in SNCA and FGF-20. Transient transfection of SH-SY5Y cells with miR-7 and miR-433 mimics resulted in down regulation of SNCA and FGF-20 mRNA levels. Our study is the first to uncover the potential link between manganese exposure, altered miRNA expression and parkinsonism: manganese exposure causes overexpression of SNCA and FGF-20 by diminishing miR-7 and miR-433 levels. These miRNAs may be considered critical for protection from manganese induced neurotoxic mechanism and hence as potential therapeutic targets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 46, February 2018, Pages 94-101
Journal: Toxicology in Vitro - Volume 46, February 2018, Pages 94-101
نویسندگان
Prashant Tarale, Atul P. Daiwile, Saravanadevi Sivanesan, Reinhard Stöger, Amit Bafana, Pravin K. Naoghare, Devendra Parmar, Tapan Chakrabarti, Kannan Krishnamurthi,