|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5562504||1562699||2018||10 صفحه PDF||سفارش دهید||دانلود کنید|
- Using PBEC-ALI, a more realistic in vivo situation is established.
- We found increased inflammatory mediators and upregulated mRNA at PBEC-ALI.
- PBEC-ALI system exposed to aldehydes, correspond well with in vivo studies.
- PBEC-ALI system with exposure via air represents a novel approach.
- This study could be used in future toxicity assessment studies of inhaled agents.
The cytotoxicity of aldehydes was studied using human primary bronchial epithelial cells (PBEC) cultured at the air-liquid interface (ALI) or under submerged conditions. PBEC were exposed for 30Â min via the air phase to acrolein (0.1-1Â mg/m3), crotonaldehyde (1.5-15Â mg/m3) or hexanal (22-221Â mg/m3) or under submerged conditions to acrolein (0.1 and 0.2Â mg/L), crotonaldehyde (1 and 2Â mg/L) or hexanal (10 and 20Â mg/L). Cell culture medium was collected 8Â h and 24Â h post-exposure and analyzed for interleukin-8 (IL-8) and matrix metalloprotein-9 (MMP-9). The gene expression of inflammatory and oxidative stress markers were measured 6Â h post-exposure. In the ALI setup, all three aldehydes caused increased secretion of IL-8, acrolein and crotonaldehyde also increased the gene expression of inflammatory and oxidative stress markers. In contrast, exposure under submerged conditions resulted in significantly reduced IL-8 secretion. The inflammatory response seen in the air phase exposures correspond well with previous in vivo studies. This indicates that lung models cultured at ALI are more suitable than submerged cell cultures in toxicity assessment studies of inhaled agents.
Journal: Toxicology in Vitro - Volume 46, February 2018, Pages 219-228