کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5562699 1562705 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Persistent organic pollutants alter DNA methylation during human adipocyte differentiation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Persistent organic pollutants alter DNA methylation during human adipocyte differentiation
چکیده انگلیسی


- TCDD and PFOS inhibited lipid accumulation in differentiating adipocytes.
- TCDD, PFOS and TBT affected DNA methylation in differentiating adipocytes.
- Differential methylation was primarily found in intergenic regions and the gene body.
- DNA methylation changes could not be associated with differential gene expression.

Ubiquitous persistent organic pollutants (POPs) can accumulate in humans where they might influence differentiation of adipocytes. The aim of this study was to investigate whether DNA methylation is one of the underlying mechanisms by which POPs affect adipocyte differentiation, and to what extent DNA methylation can be related to gene transcription. Adipocyte differentiation was induced in two human cell models with continuous exposure to different POPs throughout differentiation. From the seven tested POPs, perfluorooctanesulfonic acid (PFOS) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) decreased lipid accumulation, while tributyltin (TBT) increased lipid accumulation. In human mesenchymal stem cells (hMSCs), TCDD and TBT induced opposite gene expression profiles, whereas after PFOS exposure gene expression remained relatively stable. Genome-wide DNA methylation analysis showed that all three POPs affected DNA methylation patterns in adipogenic and other genes, possibly related to the phenotypic outcome, but without concomitant gene expression changes. Differential methylation was predominantly detected in intergenic regions, where the biological relevance of alterations in DNA methylation is unclear. This study demonstrates that POPs, at environmentally relevant levels, are able to induce differential DNA methylation in human differentiating adipocytes.

212

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 40, April 2017, Pages 79-87
نویسندگان
, , , ,