کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5584928 1567998 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fatty acid and lipid profiles in primary human trophoblast over 90 h in culture
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Fatty acid and lipid profiles in primary human trophoblast over 90 h in culture
چکیده انگلیسی
Little is known about the mechanisms underlying the preferential transport of long chain polyunsaturated fatty acids (LCPUFA) to the fetus by the syncytiotrophoblast and the role of cytotrophoblasts in placental lipid metabolism and transport. We studied primary human trophoblast (PHT) cells cultured for 90 h to determine the fatty acid and lipid composition of cytotrophoblast (18 h culture) and syncytiotrophoblast (90 h culture) cells. In cultured PHT total lipid fatty acids were significantly (P < 0.05) reduced at 90 h compared to 18 h in culture including lower levels of palmitic acid (PA, 16:0, −37%), palmitoleic acid (POA, 16:1n-7, −30%), oleic acid (OA, 18:1n-9, −31%), LCPUFA arachidonic acid (AA, 20:4n-6, −28%) and α-linolenic acid (ALA, 18:3n-3, −55%). In major lipid classes, OA and most of the n-3 and n-6 LCPUFA were markedly lower at 90 h in TG (−57 to −76%; p < 0.05). In the cellular NEFA, n-6 LCPUFA, dihomo-γ-linolenic acid (DGLA, 20:3n-6) and AA were both reduced by −51% and DHA was −55% lower (p < 0.05) at 90 h. In contrast, phospholipid FA content did not change between cytotrophoblasts and syncytiotrophoblast except for OA, which decreased by −62% (p < 0.05). Decreasing PHT TG and NEFA lipid content at 90 h in culture is likely due to processes related to differentiation such as alterations in lipase activity that occur as cytotrophoblast cells differentiate. We speculate that syncytiotrophoblast prioritizes PL containing AA and DHA for transfer to the fetus by mobilizing FA from storage lipids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - Volume 121, June 2017, Pages 14-20
نویسندگان
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