کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589384 | 1569799 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polysaccharides isolated from Hedyotis diffusa inhibits the aggressive phenotypes of laryngeal squamous carcinoma cells via inhibition of Bcl-2, MMP-2, and μPA
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کلمات کلیدی
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromideHedyotis diffusaDEAELPSHDPuPASDSMMP-2FBSTCMSCCBSA - BSADMSO - DMSOMTT - MTTbovine serum albumin - آلبومین سرم گاوdiethylaminoethyl - دی اتیل آمینو اتیلDimethyl sulfoxide - دیمتیل سولفواکسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوtraditional Chinese medicine - طب سنتی چینیlipopolysaccharide - لیپوپلی ساکاریدMetalloproteinase-2 - متالوپروتئیناز-2Migration - مهاجرتPolysaccharides - پلیساکاریدهاSquamous cell carcinoma - کارسینوم سلول سنگفرشیhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hedyotis diffusa, a traditional Chinese herbal medicine, possesses anti-cancer, anti-oxidative, and anti-inflammatory effects. The aim of this study is to explore the anti-tumor potential of Hedyotis diffusa polysaccharides (HDP) in human larynx squamous carcinoma. High performance size-exclusion chromatography analysis indicated the homogeneous nature of HDP. Total carbohydrate content in HDP was 97.3%, without contamination of protein and nucleic acid. HDP suppressed the proliferation of Hep2 human larynx squamous carcinoma cells in a time- and dose-dependent manner. Cell cycle analysis revealed that exposure to HDP (400 μg/ml) caused a G0/G1 cell cycle arrest. Moreover, treatment with HDP for 24 h induced a significant apoptosis of Hep2 cells, which was accompanied by increased cleavage of caspase-3, caspase-8, and caspase-9 and reduced expression of Bcl-2 protein. Additionally, HDP inhibited cell migration and suppressed the expression of MMP-2 and μPA. In conclusion, HDP shows suppressive effects against the aggressive phenotypes of human larynx squamous carcinoma cells and may have therapeutic potential for this malignancy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Brought to you by:GAYATRI VIDYA PARISHAD COLLEGE OF ENGINEERING for Women Renewal due by 31 Dec 2017
Journal: Gene - Brought to you by:GAYATRI VIDYA PARISHAD COLLEGE OF ENGINEERING for Women Renewal due by 31 Dec 2017
نویسندگان
Caiqin Wu, Huanan Luo, Weijun Ma, Xiaoyong Ren, Chuangxin Lu, Ni Li, Zhenghui Wang,