کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5589441 | 1569797 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Toll-receptor 9 gene in the black tiger shrimp (Penaeus monodon) induced the activation of the TLR-NF-κB signaling pathway
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کلمات کلیدی
ECDPAMPTLRPRRLRRInnate immunity - ایمنی ذاتیInvertebrates - بی مهرگانleucine-rich repeat - تکرار غنی از لوسینTIR - تیرToll-like receptor - تیالآرextracellular domain - دامنه خارج سلولیSubcellular localization - محلی سازی سلولPenaeus monodon - میگوی ببری بزرگpathogen associated molecular patterns - پاتوژن الگوی مولکولی مرتبط استToll/interleukin-1 receptor - گیرنده Toll / interleukin-1pathogen recognition receptors - گیرنده های تشخیص پاتوژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Toll receptors are important pathogen recognition receptors (PRRs) in shrimps, which play a vital role in defending against virus and bacterial challenge. In this paper, the characterization and functional analysis of a Toll9 receptor gene from Penaeus monodon was performed in HEK293T cells. Data showed that PmToll9 can activate the NF-κB promoter activities of TLR pathway, while ISRE and IFN-β promoter cannot be activated obviously in HEK293T cells using dual-luciferase reporter system. The downstream immune factors of IL-8, IκB-α, and TRAF6 were activated by PmToll9 and IL-8 showed the most significant up-regulation in expression levels, indicating the activities of NF-κB can be mediated by PmToll9. Six LRRs-deletion mutants were constructed and results showed these mutants had obvious declines in luciferase activities, among which the mutant pCMV-DeLRR4 showed the most significant decline. qPCR data indicated LRRs-deletion mutants efficiently impaired the activities of the downstream immune factors IL-8, IκB-α, and TRAF6. It demonstrates that LRRs-deletion mutants could result in the weaken abilities of PmToll9 in signaling transduction. Overexpression of PmToll9-GFP fusion protein in Hela cells revealed the primary cellular localization of PmToll9 is in the cytoplasm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 639, 10 January 2018, Pages 27-33
Journal: Gene - Volume 639, 10 January 2018, Pages 27-33
نویسندگان
Qian Liu, Dan Xu, Shigui Jiang, Jianhua Huang, Falin Zhou, Qibin Yang, Song Jiang, Lishi Yang,