کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5592140 1570706 2017 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections
ترجمه فارسی عنوان
مشخصات پروتئومیک کمی برای روشن شدن نقش حیاتی لیزوزوم در عفونت های میکروبی
کلمات کلیدی
لیزوزوم، پروتئومیکس، هیپوکسیا، میکروب ها،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
چکیده انگلیسی


- We analyzed changes in the lysosomal proteome after microbial infection.
- We used a combination of bioinformatics and proteomics strategies.
- Changes in the lysosomal proteome varied with infection by diverse microbes.
- This novel approach is suitable for investigations of the enriched organelles.

Lysosomes play vital roles in both innate and adaptive immunity. It is widely accepted that lysosomes do not function exclusively as a digestive organelle. It is also involved in the process of immune cells against pathogens. However, the changes in the lysosomal proteome caused by infection with various microbes are still largely unknown, and our understanding of the proteome of the purified lysosome is another obstacle that needs to be resolved. Here, we performed a proteomic study on lysosomes enriched from THP1 cells after infection with Listeria monocytogenes (L.m), Herpes Simplex Virus 1 (HSV-1) and Vesicular Stomatitis Virus (VSV). In combination with the gene ontology (GO) analysis, we identified 284 lysosomal-related proteins from a total of 4560 proteins. We also constructed the protein-protein interaction networks for the differentially expressed proteins and revealed the core lysosomal proteins, including SRC in the L. m treated group, SRC, GLB1, HEXA and HEXB in the HSV-1 treated group and GLB1, CTSA, CTSB, HEXA and HEXB in the VSV treated group, which are involved in responding to diverse microbial infections. This study not only reveals variable lysosome responses depending on the bacterial or virus infection, but also provides the evidence based on which we propose a novel approach to proteome research for investigation of the function of the enriched organelles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 87, July 2017, Pages 122-131
نویسندگان
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