کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5637857 1583270 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of the RPL34 gene on the growth and metastasis of oral squamous cell carcinoma cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Influence of the RPL34 gene on the growth and metastasis of oral squamous cell carcinoma cells
چکیده انگلیسی


- RPL34 gene was highly expressed in OSCC tissues and cell lines.
- Patients with positive RPL34 expression had a poorer prognosis.
- RPL34 expression was correlated with clinicopathologic features.
- Silencing RPL34 inhibited cell growth and metastasis.
- First explored the correlation between RPL34 gene and OSCC.

BackgroundOral squamous cell carcinoma (OSCC) accounts for almost 3% of all malignant tumors all over the world. This study aims to investigate the correlation of RPL34 with the cell growth and metastasis of oral squamous cell carcinoma (OSCC) as well as its clinical prognosis.MethodQuantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were conducted to determine the RPL34 expression in 85 OSCC tissues and 30 normal oral mucosa tissues. Besides, OSCC cell lines SCC-4 were divided into blank group, negative control (NC) group and RPL34-shRNA group. The qRT-PCR and western blot were performed to measure RPL34 expression, CCK-8 and flow cytometry to observe cell growth and apoptosis, and wound healing and transwell to detect cell migration and invasion.ResultsThe RPL34 gene expression was up-regulated in OSCC tissues and cells. The RPL34 expression was significantly correlated with differentiation degree, TNM stage and lymph node metastasis. Patients with positive RPL34 expression had a poorer prognosis. After inhibition of RPL34 expression, the proliferation of SCC-4 cells was slowed down at 24 h, 48 h, 72 h and 96 h respectively, and both the migration distance and the number of invasive cells were reduced, while there was an increase in the ratio of cells at G0/G1 stage and cell apoptosis.ConclusionThe RPL34 gene was highly expressed in OSCC, while silencing RPL34 could block cell proliferation and metastasis, but promote cell apoptosis, suggesting the RPL34 gene to be a new promising clinical target for OSCC therapy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 83, November 2017, Pages 40-46
نویسندگان
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