کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5638234 1583690 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Osteogenic potential of platelet-rich plasma in dental stem-cell cultures
ترجمه فارسی عنوان
پتانسیل استئوژنیک پلاسمای غنی از پلاکت در کشت سلول های بنیادی دندانی
کلمات کلیدی
بازسازی استخوان، سلول های بنیادی پالپ دندان، سلول های بنیادی لثه دندان پریودنتال، پلاسما غنی از پلاکت، فرهنگ سلولی،
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
چکیده انگلیسی

The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200 μmol; DMEM and l-ascorbate 2-phosphate 50 μmol). Cells were characterised by flow cytometry. Alizarin Red stain, alkaline phosphatase stain, and the expression of collagen type 1 (Col-1), runt-related transcription factor (RUNX2), osteoprotegerin, and osteopontin (quantified by qRT-PCR) were used to detect the osteogenic profile in each culture. Flow cytometry showed that both types of stem cell were a homogeneous mixture of CD90(+), CD105(+), STRO-1(+), CD34 (−), and CD45 (−) cells. Dental pulp stem cells that were cultured with PRP showed the best osteogenic profile (RUNX2 p = 0.0002; osteoprotegerin p = 0.001). The group of these stem cells that showed the best osteogenic profile was also cultured with PRP (osteoprotegerin p = 0.001). Medium five (with l-ascorbate 2-phosphate 50 μmol added) showed an increase in all osteogenic markers for periodontal ligament stem cells after PRP, while the best culture conditions for osteogenic expression of dental pulp stem cells after PRP was in medium four (ascorbic acid 200 μmol added). These results suggested that culture in PRP induces osteogenic differentiation of both types of stem cell, modulating molecular pathways to promote bony formation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: British Journal of Oral and Maxillofacial Surgery - Volume 55, Issue 7, September 2017, Pages 697-702
نویسندگان
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