کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5640491 | 1585463 | 2017 | 11 صفحه PDF | دانلود رایگان |
ObjectivesThe etiology of the reduced marginal bone loss observed around platform-switched implant-abutment connections is not clear but could be related to the release of variable amounts of corrosion products. The present study evaluated the effect of different concentrations of metal ions released from different implant abutment couples on osteoblastic cell viability, apoptosis and expression of genes related to bone resorption.MethodsOsteoblastic cells were exposed to five conditions of culture media prepared containing metal ions (titanium, aluminum, vanadium, cobalt, chromium and molybdenum) in different concentrations representing the amounts released from platform-matched and platform-switched implant-abutment couples as a result of an earlier accelerated corrosion experiment. Cell viability was evaluated over 21Â days using the Alamar Blue assay. Induction of apoptosis was measured after 24Â h of exposure using flow cytometry. Expression of interleukin-6, interleukin-8, cyclooxygenase-2, caspase-8, osteoprotegerin and receptor activator of nuclear factor kappa-B ligand (RANKL) by osteoblastic cells were analysed after exposure for 1, 3 and 21Â days using real-time quantitative polymerase chain reaction assayResultsMetal ions in concentrations representing the platform-matched groups led to a reduction in cell viability (PÂ <Â 0.01) up to 7Â days of exposure. Stimulated cells showed higher rates of early apoptosis (PÂ <Â 0.01) compared to non-treated cells. Metal ions up-regulated the expression of interleukin-6, interleukin-8, cyclooxygenase-2 and RANKL in a dose dependent manner after 1Â day of exposure (PÂ <Â 0.05). The up-regulation was more pronounced in the groups containing the corrosion products of platform-matched implant-abutment couples.ConclusionOsteoblastic cell viability, apoptosis, and regulation of bone resorbing mediators were significantly altered in the presence of metal ions. The change in cytokine levels expressed was directly proportional to the metal ion concentration.Clinical significanceThe observed biological responses to decreased amounts of metal ions released from platform-switched implant-abutment couples compared to platform-matched couples may partly explain the positive radiographic findings in respect to crestal bone level when utilising the “platform-switching” concept, which highlights the possible role of corrosion products in the mediation of crestal bone loss around dental implants
Journal: Journal of Dentistry - Volume 66, November 2017, Pages 91-101