کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5665572 1591179 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein carbonyl content: a novel biomarker for aging in HIV/AIDS patients
ترجمه فارسی عنوان
محتوای پروتئین کربونیل: بیومارکر جدید برای پیری در بیماران اچ آی وی / ایدز
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
چکیده انگلیسی

BackgroundThe major complications of “treated” Human Immunodeficiency Virus (HIV) infection are cardiovascular disease, malignancy, renal disease, liver disease, bone disease, and perhaps neurological complications, which are phenomena of the normal aging process occurring at an earlier age in the HIV-infected population. The present study is aimed to explore protein carbonyl content as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients.ObjectiveTo investigate the potential of carbonyl content as a biomarker for detecting oxidative Deoxyribonucleic acid (DNA) damage induced Antiretroviral Theraphy (ART) toxicity and/or accelerated aging in HIV/AIDS patients.MethodsIn this case-control study a total 600 subjects were included. All subjects were randomly selected and grouped as HIV-negative (control group) (n = 300), HIV-infected ART naive (n = 100), HIV-infected on first line ART (n = 100), and HIV-infected on second line ART (n = 100). Seronegative control subjects were age- and sex-matched with the ART naive patients and the two other groups. Carbonyl protein was determined by the method described in Levine et al. DNA damage marker 8-OH-dG was determined using 8-hydroxy-2-deoxy Guanosine StressXpress ELA Kit by StressMarq Biosciences.ResultsProtein carbonyl content levels and oxidative DNA damage were significantly higher (p < 0.05) in HIV-infected patients on second line ART and HIV-infected patients on first line ART than ART naive patients and controls. In a linear regression analysis, increased protein carbonyl content was positively associated with increased DNA damage (OR: 0.356; 95% CI: 0.287-0.426) p < 0.05.ConclusionsCarbonyl content may has a role as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients. Larger studies are warranted to elucidate the role of carbonyl content as a biomarker for premature aging in HIV/AIDS patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Brazilian Journal of Infectious Diseases - Volume 21, Issue 1, January–February 2017, Pages 35-41
نویسندگان
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