کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5665804 1407772 2017 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs
کلمات کلیدی
FINA; filtration isolation of nucleic acids; CT; Chlamydia trachomatis; HPR; hydroxypyruvate reductase; IC; internal control; qPCR; quantitative polymerase chain reaction; Cq; quantitation cycle; CI; 95% confidence interval; SP; specificity panel; Chlamyd
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
A simple and rapid DNA extraction method for Chlamydia trachomatis detection from urogenital swabs
چکیده انگلیسی

•Highly sensitive and specific Chlamydia trachomatis assay is reported.•Utilizes filtration isolation of nucleic acids (FINA) sample prep method.•Assay performance was validated with 40 clinical swab specimens.

A highly sensitive and specific Chlamydia trachomatis (CT) diagnostic test was developed by combining filtration isolation of nucleic acid (FINA) extraction with quantitative polymerase chain reaction including an internal control to identify test inhibition. A pilot study of 40 clinical specimens yielded 100% sensitivity and specificity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 89, Issue 3, November 2017, Pages 182-184
نویسندگان
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