|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5665821||1407773||2017||3 صفحه PDF||سفارش دهید||دانلود کنید|
IntroductionVariation in MRSA genotypes may affect the sensitivity of molecular assays to detect this organism.MethodsWe compared 2 commonly used screening assays, the Cepheidâ¢ Xpert® MRSA and the BD MAXâ¢ MRSA XT on consecutively obtained nasal swabs from 479 subjects. Specimens giving discordant results were subjected to additional microbiologic and molecular testing.ResultsSix hundred forty-two (97.6%) of the 658 test results were concordant. Of the 16 discordant results from 12 subjects, additional results suggested that 9 (60%) of the 15 MRSA XT assays were likely correct, and 6 (40%) of the 15 Xpert® assays were likely correct. One discordant result could not be resolved. A mecA dropout and novel mec right-extremity junction (MREJ) sites led to false-positive and negative results by Xpert®.ConclusionWhile both assays performed well, continued vigilance is needed to monitor for Staphylococcus aureus with novel MREJ sites, mecA dropouts, and mecC, leading to inaccurate results in screening assays.
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 4, April 2017, Pages 308-310