کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5665823 1407773 2017 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
کلمات کلیدی
Class A β-lactamase; Klebsiella species; Molecular identification; blaLEN; blaSHV; blaOKP;
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
چکیده انگلیسی

•A one-step and reliable diagnostic PCR for Klebsiella species identifications was proposed.•The chromosomal class A β-lactamase bla gene is a robust species marker.•The scheme robustness was determined by primers specificity and PCR reproducibility.

Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 4, April 2017, Pages 315-317
نویسندگان
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