|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|5665823||1407773||2017||3 صفحه PDF||سفارش دهید||دانلود کنید|
- A one-step and reliable diagnostic PCR for Klebsiella species identifications was proposed.
- The chromosomal class A Î²-lactamase bla gene is a robust species marker.
- The scheme robustness was determined by primers specificity and PCR reproducibility.
Klebsiella pneumoniae, Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, blaLEN and blaOKP and their flanking gene (deoR). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 4, April 2017, Pages 315-317