کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5665861 1407775 2017 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing
چکیده انگلیسی


- We developed a multiplex real-time PCR assay for detecting and typing M. pneumoniae.
- Typing was based on the presence of short type-specific indels for P1 typing.
- This assay was 100% specific compared to existing methods.

We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 3, March 2017, Pages 203-206
نویسندگان
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