کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5665873 1407775 2017 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate
کلمات کلیدی
MALDI-TOF MS; Zinc; Carbapenemase; Metallo-beta-lactamase; Gram-negative; Enterobacteriaceae;
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate
چکیده انگلیسی

•Improved testing is a vital part of the global response to carbapenemase-producing Gram-negatives.•A MALDI-TOF MS-based imipenem hydrolysis assay was enhanced with the addition of zinc ions.•This modification was necessary for the detection of some metallo-β-lactamase producing strains.•With this improvement, the assay demonstrated excellent sensitivity and specificity

A MALDI-TOF MS1-based imipenem hydrolysis assay was modified by adding ZnSO4. This improved detection of metallo-β-lactamase producing strains without compromising detection of other carbapenemase types. Using 129 genetically characterized Gram-negative bacilli, the sensitivity and specificity were 98.5% (95% confidence interval [CI]: 91.9–99.7%) and 100% (95% CI: 94.3–100%), respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 87, Issue 3, March 2017, Pages 258-260
نویسندگان
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