کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5666981 1407840 2017 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Use of whole-genome sequencing data to analyze 23S rRNA-mediated azithromycin resistance
کلمات کلیدی
Azithromycin; Resistance; Neisseria gonorrhoeae; 23S rRNA;
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Use of whole-genome sequencing data to analyze 23S rRNA-mediated azithromycin resistance
چکیده انگلیسی

•Mechanism of elevated resistance to azithromycin.•Azithromycin resistance in Neisseria gonorrhoeae analyzed by whole-genome sequencing.•Mutations in 23S rRNA.

The whole-genome sequences of 24 isolates of Neisseria gonorrhoeae with elevated minimum inhibitory concentrations (MICs) to azithromycin (≥2.0 µg/mL) were analyzed against a modified sequence derived from the whole-genome sequence of N. gonorrhoeae FA1090 to determine, by signal ratio, the number of mutant copies of the 23S rRNA gene and the copy number effect on 50S ribosome-mediated azithromycin resistance. Isolates that were predicted to contain four mutated copies were accurately identified compared with the results of direct sequencing. Fewer than four mutated copies gave less accurate results but were consistent with elevated MICs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Antimicrobial Agents - Volume 49, Issue 2, February 2017, Pages 252-254
نویسندگان
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