کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
5667162 1407849 2017 3 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Direct detection of Anaplasma phagocytophilum by polymerase chain reaction followed by electrospray ionization mass spectrometry from human blood
کلمات کلیدی
Tick-borne disease; Anaplasma phagocytophilum; HGA; IRIDICA; PCR/ESI-MS;
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Direct detection of Anaplasma phagocytophilum by polymerase chain reaction followed by electrospray ionization mass spectrometry from human blood
چکیده انگلیسی

•Anaplasmosis is difficult to diagnose as clinical symptoms are non-specific.•Anaplasma phagocytophilum is not detectable in standard blood cultures and is not covered by commercial PCR systems.•Pathogen-specific serology is mostly of value for retrospective evaluation, as it lacks diagnostic accuracy at early patient presentation.•The newly available PCR/ESI-MS system demonstrated the presence of A. phagocytophilum in the peripheral blood of a febrile patient.•PCR/ESI-MS represents a promising novel diagnostic methodology for the rapid assessment of rare or exotic pathogens.

SummaryBacterial pathogens not detectable via commercial blood culture assays represent an important challenge for infectious disease physicians, in particular if clinical symptoms of the illness are non-specific. In this report, Anaplasma phagocytophilum was detected directly in a peripheral blood sample from a febrile patient reporting a tick bite. This was done using a commercial system based on PCR followed by electrospray ionization mass spectrometry (ESI-MS). The diagnosis of a human granulocytic anaplasmosis infection was established using this diagnostic methodology for the first time. Human granulocytic anaplasmosis is a neglected zoonotic disease in Europe. Its seroprevalence is similar in North America and Europe, but in contrast to the USA, it is rarely diagnosed in the old world. PCR followed by ESI-MS is a novel, complex, but highly promising diagnostic methodology for the rapid assessment of rare or exotic pathogens, including intracellular bacteria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Infectious Diseases - Volume 60, July 2017, Pages 61-63
نویسندگان
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