کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5667962 1592330 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rotavirus genotype shifts among Swedish children and adults-Application of a real-time PCR genotyping
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Rotavirus genotype shifts among Swedish children and adults-Application of a real-time PCR genotyping
چکیده انگلیسی


- New real-time PCR genotyping method for rotavirus.
- Presented method genotyped 97.3% of rotavirus regarding both VP4 and VP7.
- Rotavirus genotype distribution change over time.
- Genotype distribution is related to age and differ between age groups.

BackgroundIt is well known that human rotavirus group A is the most important cause of severe diarrhoea in infants and young children. Less is known about rotavirus infections in other age groups, and about how rotavirus genotypes change over time in different age groups.ObjectivesDevelop a real-time PCR to easily genotype rotavirus strains in order to monitor the pattern of circulating genotypes.Study designIn this study, rotavirus strains in clinical samples from children and adults in Western Sweden during 2010-2014 were retrospectively genotyped by using specific amplification of VP 4 and VP 7 genes with a new developed real-rime PCR.ResultsA genotype was identified in 97% of 775 rotavirus strains. G1P[8] was the most common genotype representing 34.9%, followed by G2P[4] (28.3%), G9P[8] (11.5%), G3P[8] (8.1%), and G4P[8] (7.9%) The genotype distribution changed over time, from predominance of G1P[8] in 2010-2012 to predominance of G2P[4] in 2013-2014. There were also age-related differences, with G1P[8] being the most common genotype in children under 2 years (47.6%), and G2P[4] the most common in those over 70 years of age (46.1%.). The shift to G2P[4] in 2013-2014 was associated with a change in the age distribution, with a greater number of rotavirus positive cases in elderly than in children.ConclusionsBy using a new real-time PCR method for genotyping we found that genotype distribution was age related and changed over time with a decreasing proportion of G1P[8].

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 96, November 2017, Pages 1-6
نویسندگان
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