کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5668072 1592335 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
EBV-encoded microRNAs profile evaluation in pediatric liver transplant recipients
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
EBV-encoded microRNAs profile evaluation in pediatric liver transplant recipients
چکیده انگلیسی


- Stem-loop MGB real time PCR has been used to detect EBV miRNAs levels.
- EBV miR-BART2-5p, miR-BART15 and miR-BART22 were investigated.
- miR-BART22 levels in patients with positive EBV PCR were higher.
- miR-BART2-5p and miR-BART15 did not differ in positive and negative EBV PCR.
- miR-BART22 is a potential biomarker for EBV reactivation.

BackgroundEpstein-Barr virus (EBV) infects 90% of the world population, commonly causing self-limiting infectious mononucleosis or rarely inciting a range of malignancies. EBV microRNAs (miRNAs) were discovered by sequencing libraries of small RNAs generated from several EBV-positive cell lines. Little is known about their roles, but their high stability and easy quantification make these molecules potential biomarkers.ObjectivesIn this study a stem-loop MGB real-time RT-PCR has been used to detect and quantify miR-BART2-5p, miR-BART15 and miR-BART22 EBV miRNAs levels.Study designsThe profiles of EBV miRNAs levels were evaluated in 51 serum samples of 37 pediatric liver transplant patients subdivided in 3 study groups: EBV seronegative, EBV seropositive and PCR negative, EBV seropositive and PCR positive.ResultsmiR-BART22 serum levels in patients with positive EBV PCR were significantly higher than those in patients with negative EBV PCR (p = 0.0005). On the contrary, miR-BART2-5p and miR-BART15 did not exhibit significant difference in positive and negative EBV PCR patients (p = 0.5511 and p = 0.3523, respectively).ConclusionThis study described a method for quantitative detection of miR-BART 22, miR-BART2-5p and miR-BART15 EBV miRNAs in liver transplanted patients, and suggests the use of miR-BART22 as a potential biomarker for EBV reactivation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 91, June 2017, Pages 36-41
نویسندگان
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