کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5671223 1592820 2017 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional gene profiling through metaRNAseq approach reveals diet-dependent variation in rumen microbiota of buffalo (Bubalus bubalis)
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Functional gene profiling through metaRNAseq approach reveals diet-dependent variation in rumen microbiota of buffalo (Bubalus bubalis)
چکیده انگلیسی


- Functionally active rumen microbiota of 8 buffaloes was studied across three different diet treatments.
- Variations in microbiome were observed among liquid and solid fractions as well as among three diets treatments with different roughage proportion.
- Bacteroidetes and Firmicutes were the dominant phyla in the rumen of buffalo.
- Genes involved in carbohydrate metabolism were highly annotated followed by genes involved in amino acid metabolism.
- Within CAZy families, GH13 family (mostly α-amylases) was the most abundant, with highest proportion in liquid fraction of 100% roughage diet.

Recent advances in next generation sequencing technology have enabled analysis of complex microbial community from genome to transcriptome level. In the present study, metatranscriptomic approach was applied to elucidate functionally active bacteria and their biological processes in rumen of buffalo (Bubalus bubalis) adapted to different dietary treatments. Buffaloes were adapted to a diet containing 50:50, 75:25 and 100:0 forage to concentrate ratio, each for 6 weeks, before ruminal content sample collection. Metatranscriptomes from rumen fiber adherent and fiber-free active bacteria were sequenced using Ion Torrent PGM platform followed by annotation using MG-RAST server and CAZYmes (Carbohydrate active enzymes) analysis toolkit. In all the samples Bacteroidetes was the most abundant phylum followed by Firmicutes. Functional analysis using KEGG Orthology database revealed Metabolism as the most abundant category at level 1 within which Carbohydrate metabolism was dominating. Diet treatments also exerted significant differences in proportion of enzymes involved in metabolic pathways for VFA production. Carbohydrate Active Enzyme(CAZy) analysis revealed the abundance of genes encoding glycoside hydrolases with the highest representation of GH13 CAZy family in all the samples. The findings provide an overview of the activities occurring in the rumen as well as active bacterial population and the changes occurring through different dietary treatments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Anaerobe - Volume 44, April 2017, Pages 106-116
نویسندگان
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