کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5672883 1593426 2018 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ELISA for detection of variant rabbit haemorrhagic disease virus RHDV2 antigen in liver extracts
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
ELISA for detection of variant rabbit haemorrhagic disease virus RHDV2 antigen in liver extracts
چکیده انگلیسی


- An ELISA for specific detection of RHDV2 antigen has been designed and validated.
- The ELISA has high sensitivity (100%) and specificity (97.22%).
- No cross-reaction with RHDV G1 and low cross-reactivity with RHDVa viruses.
- Specific serological tool for monitoring virus circulation and controlling disease.

The emergence and rapid spread of variant of the rabbit hemorrhagic disease virus (RHDV2) require new diagnostic tools to ensure that efficient control measures are adopted.In the present study, a specific sandwich enzyme-linked immunosorbent assay (ELISA) for detection of RHDV2 antigens in rabbit liver homogenates, based on the use of an RHDV2-specific monoclonal antibody (Mab) 2D9 for antigen capture and an anti-RHDV2 goat polyclonal antibody (Pab), was developed. This ELISA was able to successfully detect RHDV2 and RHDV2 recombinant virions with high sensitivity (100%) and specificity (97.22%). No cross-reactions were detected with RHDV G1 viruses while low cross-reactivity was detected with one of the RHDVa samples analyzed. The ELISA afforded good repeatability and had high analytical sensitivity as it was able to detect a dilution 1:163,640 (6.10 ng/mL) of purified RHDV-N11 VLPs, which contained approximately 3.4 × 108molecules/mL particles.The reliable discrimination between closely related viruses is crucial to understand the epidemiology and the interaction of co-existing pathogens. In the work described here we design and validate an ELISA for laboratory based, specific, sensitive and reliable detection of RHDVb/RHDV2. This ELISA is a valuable, specific virological tool for monitoring virus circulation, which will permit a better control of this disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 251, January 2018, Pages 38-42
نویسندگان
, , , , , , , , , , , , ,