کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5673668 1593679 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro antiviral efficacy of caffeic acid against canine distemper virus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
In vitro antiviral efficacy of caffeic acid against canine distemper virus
چکیده انگلیسی


- The antiviral activity of the caffeic acid against CDV was evaluated in vero cells.
- The caffeic acid inhibits viral RNA accumulation during CDV replication cycle.
- The caffeic acid plus RBV inhibited the viral intracellular and extracellular stage of replicative cycle.

Canine distemper (CD) is a highly contagious disease caused by the canine distemper virus (CDV), and mortality can be as high as 100%. However, there is no specific treatment for CD. In this study, the antiviral activity of the caffeic acid against CDV was evaluated in vitro. The results showed that the IC50 of the caffeic acid against CDV at 1 and 2 h post infection (PI) is 23.3 and 32.3 μg/mL, respectively. Consistently, at 1 and 2 h PI, the caffeic acid exhibited a reduced (23.3-57.0% and 37.2-38.1%) viral inhibitory effect in vero cells. Furthermore, the caffeic acid plus Ribavirin (RBV) has greater antiviral activity against CDV than the caffeic acid or RBV individually. In addition, the caffeic acid reduced the total viral RNA synthesis by 59-86% at 24-72 h. Therefore, our data provided the experimental evidence that the caffeic acid effectively inhibited CDV infection in vero cells, which may potentially be used to treat clinical disease associated with CDV infection.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 110, September 2017, Pages 240-244
نویسندگان
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