کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5675331 | 1594322 | 2017 | 6 صفحه PDF | دانلود رایگان |
- Incorporation of miR-155 into the influenza A virus genome highlights the potential of this technology to create self-adjuvanting live vaccines.
- miRNA-155 expression enhanced influenza-specific CD8+ T cell responses.
- miRNA-155 expression enhanced influenza-specific antibody responses.
Influenza A vaccine efficacy in the elderly is generally poor and so identification of novel molecular adjuvants to improve immunogenicity is important to reduce the overall burden of disease. Short non-coding RNAs, known as microRNAs (miRNAs) are known to regulate gene expression and have the potential to influence immune responses. One such miRNA, miR-155, has been shown to modulate T and B cell development and function. We incorporated miR-155 into the influenza A virus (IAV) genome creating a self-adjuvanting 'live vaccine' with the ability to modify immunogenicity. Infection of mice with a recombinant influenza virus encoding miR-155 in the NS gene segment altered epitope-specific expansion of influenza-specific CD8+ T cells and induced significantly higher levels of neutralising antibody.
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Journal: Virus Research - Volume 235, 2 May 2017, Pages 115-120