کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5675512 | 1594318 | 2017 | 13 صفحه PDF | دانلود رایگان |
- NGS improves ART outcome predictions in ARV-naïve subjects starting NNRTIs and CCR5 antagonists.
- NGS might also be helpful to select salvage ART regimens in ART-experienced subjects.
- Transmission of INSTI remains anecdotal; Integrase genotyping is not currently mandatory before initiating INSTIs.
- In a scenario where global HIV-1 eradication is now considered possible, making NGS accessible also to LMICs is a priority.
- Reductions in NGS costs and increased accessibility to simplified bioinformatic NGS data analysis remain essential to end the HIV-1 pandemic.
The emerging HIV-1 resistance epidemic is threatening the impressive global advances in HIV-1 infection treatment and prevention achieved in the last decade. Next-generation sequencing is improving our ability to understand, diagnose and prevent HIV-1 resistance, being increasingly cost-effective and more accessible. However, NGS still faces a number of limitations that need to be addressed to enable its widespread use. Here, we will review the main NGS platforms available for HIV-1 diagnosis, the factors affecting the clinical utility of NGS testing and the evidence supporting âor not- ultrasensitive genotyping over Sanger sequencing for routine HIV-1 diagnosis. Now that global HIV-1 eradication might be within our reach, making NGS accessible also to LMICs has become a priority. Reductions in sequencing costs, particularly in library preparation, and accessibility to low-cost, robust but simplified automated bioinformatic analyses of NGS data will remain essential to end the HIV-1 pandemic.
Journal: Virus Research - Volume 239, 15 July 2017, Pages 69-81