کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5718201 1411244 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intestinal epithelial injury induced by maternal separation is protected by hydrogen sulfide
ترجمه فارسی عنوان
آسیب اپیتلیال روده ای ناشی از جداسازی مادر توسط سولفید هیدروژن محافظت می شود
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پریناتولوژی (پزشکی مادر و جنین)، طب اطفال و بهداشت کودک
چکیده انگلیسی

PurposeOxidative stress has been implicated in the pathogenesis of various neonatal diseases involving the intestine. Hydrogen sulfide (H2S) has been shown to protect against oxidative stress. We hypothesized that administration of sodium hydrosulfide (NaHS), an H2S donor, to neonatal mice can decrease the intestinal epithelial injury associated with maternal separation (MS).MethodsC57BL/6 mice received either intraperitoneal phosphate buffered saline (PBS; n = 10) or NaHS (1 mg/kg/day; n = 10), followed by MS for 3 h daily between postnatal day P5 and P9. Control neonatal mice were untreated and were not exposed to MS (n = 10). Proximal colon was harvested and analyzed for crypt length, goblet cell number per crypt, oxidative stress and inflammation. Groups were compared using one-way ANOVA with Bonferroni post-test.ResultsCompared to controls, MS + PBS mice had shorter crypt lengths, fewer goblet cells per crypt, reduced glutathione peroxidase activity, increased expression of thiobarbituric acid reactive substances and inducible nitric oxide synthase mRNA, as well as increased IL-6, TNFα and myeloperoxidase. Administration of NaHS significantly counteracted these negative effects of MS.ConclusionsH2S protects the colon from the epithelial damage, oxidative stress and inflammation caused by maternal separation. This study provides insights on the pathogenesis of neonatal bowel diseases and indicates the potential for a pharmacological intervention to rescue the colonic epithelium.Level of evidencen/a - animal and laboratory study.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pediatric Surgery - Volume 52, Issue 1, January 2017, Pages 40-44
نویسندگان
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