کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5738455 1615051 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Research articleKnockdown of APPL mimics transgenic Aβ induced neurodegenerative phenotypes in Drosophila
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Research articleKnockdown of APPL mimics transgenic Aβ induced neurodegenerative phenotypes in Drosophila
چکیده انگلیسی


- APPL-RNAi mimics Aβ induced phenotypes like eye degeneration, reduced longevity and motor deficit functions.
- Neuronal tissue specific loss of APPL show reduced longevity comparable to Aβ expressing flies.
- APPL is required for motor neuron activity and eye development in Drosophila.

A variety of Drosophila mutant lines have been established as potential disease-models to study various disease mechanisms including human neurodegenerative diseases like Alzheimer's disease (AD), Huntington's disease (HD) and Parkinson's disease (PD). The evolutionary conservation of APP (Amyloid Precursor Protein) and APPL (Amyloid Precursor Protein-Like) and the comparable detrimental effects caused by their metabolic products strongly implies the conservation of their normal physiological functions. In view of this milieu, a comparative analysis on the pattern of neurodegenerative phenotypes between Drosophila APPL-RNAi line and transgenic Drosophila line expressing eye tissue specific human Aβ (Amyloid beta) was undertaken. Our results clearly show that Drosophila APPL-RNAi largely mimics transgenic Aβ in various phenotypes which include eye degeneration, reduced longevity and motor neuron deficit functions, etc. The ultra-structural morphological pattern of eye degeneration was confirmed by scanning electron microscopy. Further, a comparative study on longevity and motor behaviour between Aβ expressing and APPL knockdown lines revealed similar kind of behavioural deficit and longevity phenotypes. Therefore, it is suggested that APPL-knockdown approach can be used as an alternative approach to study neurodegenerative diseases in the fly model. To the best of our knowledge this is the first report showing comparable phenotypes between APPL and Aβ in AD model of Drosophila.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 648, 1 May 2017, Pages 8-13
نویسندگان
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