کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5741008 1616983 2017 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Full length articleAdhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis
ترجمه فارسی عنوان
طول کامل مقاله مولکولهای واکنش، شیمیایی و پاسخ متال پروتئیناز ماتریکس پس از آلبندازول و آلبندازول به همراه استروئید درمانی در عصبی مرکزی خوکی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
چکیده انگلیسی


- Albendazole (ABZ) therapy was more effective than Albendazole plus steroid (ABZS).
- ABZ was not safe in case of heavy cysts infection (≥10) in the brain.
- ABZ responder cysts induced adhesion molecules, chemokines and MMPs expression.
- However, ABZS therapy induced the moderate immune response in responder cysts.
- ABZS non-responder cysts were associated with lower response of above molecules.

The treatment of neurocysticercosis (NCC) varies with location, number and stage of the Taenia solium cysticerci (cysts). Albendazole (ABZ) effectively kills cysticerci, and subsequently induces neuro-inflammation facilitated by leukocyte infiltration. We hypothesize that immune response varies around drug responder (degenerating/dying) and non-responder (viable) cysts after ABZ and ABZ plus steroid (ABZS) therapy, which may determine the disease pathogenesis. Twenty cysticercotic swine were treated with ABZ (n = 10; group1) and ABZS (n = 10; group2). Expression of adhesion molecules, chemokines and matrix metallo-proteinases (MMPs) was measured by qRT-PCR (quantitative reverse transcriptase-polymerase chain reaction) and ELISA. Gelatin gel zymography was performed to detect the activity of MMP-2 and -9. In group1, ABZ therapy induced higher expressions of ICAM-1 (intercellular adhesion molecule-1), VCAM-1 (vascular cell adhesion molecule-1), E-selectin, MCP-1 (monocyte chemotactic protein-1), Eotaxin-1, MIP-1α (macrophage inflammatory protein-1α), RANTES (regulated on activation, normal T cell expressed and secreted), MMP-2 and MMP-9 around ABZ responder (AR) cysts. Three pigs with cyst burdens ≥10 died following ABZ therapy. However, in group2, moderate expressions of ICAM-1, VCAM-1, E-selectin, RANTES and MMP-9 were associated with ABZS responder (ASR), whereas low expressions of these molecules were associated with ABZS non-responder (ASNR) cysts. In conclusion, ABZ alone therapy is not safe since it causes death of pigs due to higher inflammatory immune response around dying cysts. However, combination therapy is an effective treatment regimen even with the high cyst burden.

219

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 182, November 2017, Pages 1-8
نویسندگان
, , , , , , ,