کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5741111 1616988 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Detection of Leishmania infantum DNA in conjunctival swabs of cats by quantitative real-time PCR
چکیده انگلیسی


- We compared the efficacy of qPCR and cPCR for Leishmania spp. detection in blood and conjunctival swab (CS) of cats.
- Of CS samples, 1.85% were Leishmania spp. positive by cPCR and qPCR, indicating excellent agreement between the methods.
- CS tested by real-time PCR can be a useful tool to help studies about Leishmania infection and disease in cats.

Although some studies have investigated the potential role of cats as a reservoir for Leishmania, their role in the epidemiology of visceral leishmaniasis (VL) is still poorly understood. Molecular diagnostic techniques are an important tool in VL diagnosis, and PCR shows high sensitivity and specificity for Leishmania spp. detection. Quantitative real-time PCR (qPCR) is a method that permits quantitative analysis of a large number of samples, resulting in more sensitive, accurate, and reproducible measurements of specific DNA present in the sample. This study compared real-time PCR (qPCR) and conventional PCR (cPCR) for detection of Leishmania spp. in blood and conjunctival swab (CS) samples of healthy cats from a non-endemic area in the state of São Paulo, Brazil. Of all CS samples, 1.85% (2/108) were positive for Leishmania spp. by both cPCR as qPCR (kappa index = 1), indicating excellent agreement between the two methods. The DNA from the two CS-cPCR- and CS-qPCR-positive samples was further tested with a PCR test amplifying the Leishmania spp. discriminative rRNA internal transcribed spacer 1 (ITS 1), of which one sample generated a 300-350-bp DNA fragment whose size varies according to the Leishmania species. Following sequencing, the fragment showed 100% similarity to a GenBank L. infantum sequence obtained from a cat in Italy. In conclusion, the association of qPCR and CS proved to be effective for detection of Leishmania in cats. Conjunctival swab samples were shown to be a practical and better alternative to blood samples and may be useful in the diagnosis and studies of feline leishmaniasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 177, June 2017, Pages 93-97
نویسندگان
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