Effects of Fe(III) and quality of humic substances on As(V) distribution in freshwater: Use of ultrafiltration and Kohonen neural network

- Evidence of associations of As(V)-Fe(III)-HS by ultrafiltration studies.
- Most As, DOC and Fe appeared in the higher molecular size fraction of HS.
- Different behaviour of HS toward As complexation in relation to HS quality.
- Kohonen neural network enabled the detection of particular characteristics of HS.

Humic substances (HS) are ubiquitous organic compounds able to affect mobility and availability of arsenic (As) in aquatic systems. Although it is known that associations between HS and As occur mainly via iron (Fe)-cationic bridges, the behaviour and distribution of this metalloid in HS- and Fe-rich environments is still not fully understood. In this paper, the quality of HS from different rivers in Brazil and Germany and its influence on the behaviour of As(V) under different Fe(III) concentrations were investigated. HS were extracted from four different rivers (Cascatinha, Holtemme, Selke and Warme Bode), characterised and fractionated into different molecular weight sizes (10, 5 and 1 kDa). Complexation tests were performed using an ultrafiltration system and 1 kDa membranes. All data was analysed using the Kohonen neural network (SOM - Self organising maps). All samples, except Selke, exhibited similar results of free As (<1 kDa). The results suggested that associations between HS, Fe and As were dependent on nitrogen (N)-aromatic carbon (C), amount of sulphur (S) and the molecular size of the HS. Although all HS appeared to be similar after looking at most variables analysed, the SOM could discriminate them into three different groups. Characterisation of the HS indicated that they had terrestrial material (from C3 plants) as precursor material. Most of the As and Fe was distributed in the fractions of higher (>10 kDa) and lower (<1 kDa) size. HS quality is an important factor to take into account when studying the behaviour of As in HS-rich environments.

222